- Runs in Windows (XP, Vista, 7, 8, and 10) and Mac (OS X v10.5 and above)
- Highlights restriction sites in the editing window
- Accurately reflects Dam/Dcm blocking of enzyme sites
- Highlights text using pre-defined and custom feature libraries
- Shows translation, Tm, %GC, ORF of selected DNA in real-time
- Reads DNA Strider, Fasta, Genbank and EMBL files
- Saves files as DNA Strider-compatible or Genbank file format
- Highlights and draws graphic maps using feature annotations from genbank and embl files
- Directly BLASTs selected sequence at NCBI or wormbase
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Sep 10, 2007. ApE is an easy to use plasmid editor application for Mac. Main features: - Highlights restriction sites in the editing window. Accurately reflects Dam/Dcm blocking of enzyme sites. Highlights text using pre-defined and custom feature libraries. Shows translation. For all Mac users: There is a severe bug in the latest OS versions (Yosemite and later, I think). When you double-click to open a file, if ApE is already running it will crash. I'm working on fixing this. Until a fix is found, you can work around the issue by only opening files from the FileOpen dialog. Sep 15, 2011.
- Text map shows DNA sequence, translation, and features as text-based graphics
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Ape Plasmid Software
- Creates graphic restriction maps- linear or circular with features indicated
- Connects graphic and text features with hyperlink double click
- Saves graphics as encapsulated postscript or scalable vector graphics
- Copy and save graphics as Windows metafiles (MS Windows only)
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- Virtual restriction digest
- Draws pre-defined and user-defined DNA ladders
- Connects bands to text by double-click
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- Reads ABI sequencing trace files
- Sequences in ABI traces can be aligned directly to a reference sequence, with the alignment hyperlinked back to te trace.
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- Selects sites matching multiple criteria (union/intersection- cut frequency, site type) in all open windows
- Selects sites that cut more often in one sequence than another (for snip-SNP detection or diagnostic digests)
- Has user defined enzyme grouping to distiguish eg. enzymes currently in stock.
- Allows users to define new enzymes by name and recognition site
- Imports DNA Strider format files (simple enzyme, site lists) available from REBASE
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Another Plasmid Editor
Other Features: | - Most analysis windows are hyperlinked to their corresponding sequences, including:
- Graphic Maps
- Text maps
- Virtual Digests
- Alignments (including ABI sequences)
- Silent Sites
- Translation
- Primer Find
- Uses custom feature definition libraries, which allow:
- Quick annotation of sequence
- Quick searching and highlighting of all available primers that you (or others) have that hybridize to a sequence
- Sequence to be annotated and visualized in multiple ways quickly and efficiently
- Graphic maps that show primer binding sites and all interesting sequence features
- Translates sequences with optional DNA alignment
- Finds potential primers matching user criteria (length, Tm, %GC, self/other complementarity)
- Aligns two DNA sequences (or any combination of sequence and ABI trace), with the alignment hyperlinked to the original sequence
- Finds translationally silent restriction sites
- Draws graphic ORF maps
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